1 (GraphPad Software Inc. SoftMax Pro Software, data can be visualized as gray scale, kinetic plots or reaction rates. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. 5. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. 3 Pages. 4 million by 2027; it is expected to grow at a CAGR of 3. 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). The Hidex Sense is a compact, state-of-the-art, multi-mode microplate reader which provides a powerful and flexible platform for performing any kind of assay without limitations. Download Table of Contents. Besides the number of substrates used by microbiota,. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by. Biofilm quantification and scanning electron microscopy (SEM) analysis. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. It allows the user to choose between monochromator-based optics for flexibility or filter-based optics for sensitivity. FilterMax F3 & F5 Microplate Readers. Absorbance microplate assays For more than 30 years, absorbance-based detection has been the preferred mode for many microplate-based assays, such as ELISA, protein, and nucleic acid quantitation or enzymatic assays. $3,000 USD. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. shopping cart 0 items $0. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. ] Example of document to evaluate if the reader . At least three independent experiments were performed for each. The papers, which were published between 2010 and. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. The colorimetric signal development was stopped after 10 minutes. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The EMax Plus measures flat and round 96-well microplates. To read the absorbance, a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA 95134, USA) with a wavelength of 595 nm was used. performs within its specifications. 00 USD. Caspase 3/7 activity was performed as previously described [8,9,10,20]. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Firefly and Renilla luciferase relative light units were measured using the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). By category. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance. SpectraMax Glo Steady-Luc Reporter Assay Kit. F3/F5 Multi-Mode Microplate ReadersThe plate was incubated for 1 hr at room temperature with shaking. 2 Pages. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. Neurotransmitter Transporter Uptake Assay Kit. $3,000 USD. Page: 1/4NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. Recommended Optics P/N Filter information 6590-0048 FilterMax™ Fluorescence Excitation Filter 405 nm (bandwidth 30 nm) FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate Reader Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. FilterMax F5 Multi-mode microplate reader. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. Share. Click “Eject Plate Carrier. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. CloneMatrix. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. GPCRs. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. Laser-aided direct metal deposition of metals and alloys. The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. Citations: Dual monochromators increase performance and assay flexibility. After the mixture was cooled, TBARS was measured at 532 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Reader, Molecular Devices). Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. Protein contamination can be measured at 280 nm. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Bioprocess Equipment and Technology. shopping cart 0 items $0. Transfluor Technology. DMSO was used to dissolve the compounds and was included in the microplate at 0. Search LabX. SpectraMax® iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. MetaMorph NX. F3/F5 Multi-Mode Microplate Readers. 00. S. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. Absorbance Detection Comparison Table. 5. (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. Thermo Fisher Scientific. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. A Vision for the Future With TTP LabTech. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. USD ($) USD - United States Dollar. This microplate reader also features BioTek’s Hybrid Technology, which. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Sunnyvale, Calif. Unsuccessful migration. Readings at 575 nm were subtracted from the readings at 450 nm to correct for optical imperfections in the plate. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Description: The FilterMax™ F3 features absorbance (visible),. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. FilterMax F5 Multi–Mode microplate reader from Molecular Devices Multi–Mode Analysis software. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. In addition, the FilterMax F5 Reader is capable of measuring nucleic acids directly at 260 nm in absorbance detection mode. Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. F5 Multi-Mode Microplate Reader. FilterMax F5 Multi-Mode Microplate Reader. 5. Molecular Devices technical support is available by phone and e-mail. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. To contact technical support by phone in the U. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. 5 million in 2019 and is projected to reach US$ 704. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. 00 SpectraMax M3, M4, M5, and M5e Multi-Mode Document type: User Manual5016208D 8 Chapter1:Introduction TheFilterMax™ F3Multi-ModeMicroplateReaderandtheFilterMax™F5Multi-Mode MicroplateReaderareidealforabroadrangeofapplications. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). Depending on the model, Cytation 5 detection modes include fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), luminescence, UV-visible absorbance, imaging, and alpha. 8 Pages. The haemolytic activity was determined in the cell supernatant by optical density readings at 570 nm (FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices). With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The measurement can be performed using a variety of detection technologies: the. MS-3 Laser Bar Code Reader. The increase in NADH, an end product in this reaction, was determined by. FilterMax F3 & F5 Microplate Readers. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. 3 Pages. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. FilterMax F3 & F5 Multi-Mode Microplate Readers from. SpectraMax Quant dsDNA Assay Kits. 561. After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. 50/50. This document is provided to customers. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. prt Read the plate and then save the experiment. Add to my manuals. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. 400. HERM LB 500 with NaI detector Radio HPLC Detector. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 405 was measured in a FilterMax™ F5 Multi-Mode Microplate Reader. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. Match case Limit results 1 per page. 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. , Sunnyvale, CA, USA). Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. After adding 100 μl of new ice-cold PBS to the cells, fluorescent intensity was immediately determined using the fluorescent plate reader (FilterMax F3&F5 Multi-Mode Microplate Reader, Molecular Devices). After 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seF3/F5 Multi-Mode Microplate Readers. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. 1. Time-resolved Fluorescence. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#: STEM-LE-0509-LC) Mithras LB 940 Multimode Microplate Reader, 200-1000 nm Spectral Range Absorbance, BERTHOLD TECHNOLOGIES GmbH & Co. The. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. Plaque reduction. 4 Pages. The amount of colorimetric product was continuously monitored for 120 min. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Cytokine concentration was determined by plotting the OD or median fluorescence intensity (MFI) of each sample on each cytokine concentration curve and multiplying the result by a dilution. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and. 003 fmol 0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 4 Pages. Add to cart. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. aureus , the amount of bacteriophage-MBs and the dilution ratio of the HRP. 3D cell proliferation assays were conducted using high concentrations to optimize conditions for cell lines in 20, 100 or• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Time-resolved Fluorescence. The quantity of colorimetric product was continuously monitored for 120 min. MetaFluor Software. IMAP assays are based on binding of. 020 fmol 0. SpectraMax Gemini XPS/EM Microplate Readers. OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader with high sensitivity. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. Neurotransmitter Transporter Uptake Assay Kit. ” e. BMG Labtech - THERMOstar. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. The OD value of each tube (230 μL) was measured in a 96-well microplate at 405 nm using a. 8 Pages. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. microplate reader. The standard curve presented a linear phase from 1. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 11 mg/mL in PBS, 20 h incubation) using the FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices (Sunnyvale, CA, USA), at 570 nm . The ucs load command creates a backup of. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). Assay buffer and 100% DMSO were used as vehicle controls. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Table of Contents. com 8. Serum concentrations of CAF were determined according to manufacturer guidelines in duplicate via enzyme-linked immunosorbent assay (Glory Science Co. The amount of each HTRA1 protein was. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Optical System: High energy xenon flash lamp. Products : General, FilterMax F5 reader. 7°C (gradient heating design). F5 Multi-Mode Microplate Reader. Supernatant (25 μL), or standard, was added and mixed. . Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. FilterMax F3 / FilterMax F5: Inquire: SpectraMax iD3 and iD5: SpectraMax M: Price: Description: Unlimited breadth of user-upgradeable application modules expands research capabilities. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. 3 Pages. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Bio-Tek Microplate Reader Synergy HT $8,995. HTRF-compatible SpectraMax Multi-Mode Readers. CloneMatrix. A FilterMax F5 multi-mode microplate reader (Molecular Devices, San Jose, USA) was used to determine the absorbance (OD 450nm -OD 570nm). The donor and acceptor can be used to label a variety of biomolecules, for. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). Place plate in the GloMax® Instrument and read. 100 fmol 384-well (75 µL) 0. F3 F5 F3-SC F5-SC F3 F5. Guiberteau MD, in Essentials of Nuclear Medicine Imaging (Sixth Edition), 2012 Photon Transducers. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). 0. The following document is. 4 Pages. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). ScanLater Western Blot Detection System. Synergy HTX and Synergy LX. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. 5 min for 30 min at 340 nm absorbance using a microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, FilterMax F5 Multi-Mode Microplate Reader) and Multi-Mode Analysis Software (Molecular Devices, RRID:SCR_014789), version 3. Create an experiment based on the protocol. Categories:. Instrumentation and Quality Control. Luminescence (glow) Fluorescence polarization. 8 Pages. All experiments were performed in triplicate. Add to cart. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Neurotransmitter Transporter Uptake Assay Kit. Cells were seeded in 24-well plates and cultured. FilterMax® F3 & F5 Multi-Mode Microplate Readers. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. 3 Pages. Molecular Devices technical support. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). GenePix 4300/4400 Microarray Scanner. Each kit was used on the same day that its reagents were reconstituted. 00. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Add to my manuals. GenePix 4300/4400 Microarray Scanner. They provide absorbance, fluorescence, glow luminescence, and other read modes for Affordability and unmatched assay performance in filter-based microplate readers. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. The optical performance is comparable to a top-of-the-line dedicated Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. Devices FilterMax Multi-Mode Microplate Readers. I. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. Chemiluminescence measurements were performed using a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). For WST-1–based assay, 10 μL ready-to-use WST-1 reagent was added to each well and incubated for 3 hours at 37°C, and absorbance at 450 nm was measured. 2. Microplate formats can vary from 6-1536 wells, providing the ability to read many samples in a short time. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. FITC-conjugated casein assay substrate was added to the wells and incubated at room temperature for 2 hours. A microplate consists of small wells wherein separate reactions take place. Turn on the power switch on the back of the instrument. In addition, the FilterMax F5 Reader is capable of measuring. Other technologies such as imaging. In the list below the slide number 21, select the positions where the emission filters are installedAfter 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). Your applications, your modes, your choice. Page 154: Ordering Parts And Accessories. 425 or. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The supernatant was taken and 0. F3/F5 Multi-Mode Microplate Readers The FilterMax Series of microplate readers includes: FilterMax F3 Multi-Mode Microplate Reader. Microplate Readers. FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#:. GFP expression differences. Instrument Configuration: Assay Technology Adapta® & Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET only) (TR-FRET only) HTRF Certification LanthaScreen Certification Key. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Transfluor Technology. 3 Pages. shopping cart 0 items $0. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. Tristar 3 Multimode Microplate Reader. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. 3 Pages. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. FilterMax F5 Multi-Mode Microplate Reader. URL of this page: HTML Link: Bookmark this page. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). Learn how to set up protocols, customize reports, export results, and more with this PDF document. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. Manufacturer: Molecular Devices. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. Aspirate 150 µL from wells C5-F5 and discard. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. Multi-Mode Readers. 09 CAD. The 0. ” e. Turn on the power switch on the back of the instrument. Luminescence was recorded with a luminometer (Filter Max F5 Multi-Mode Microplate reader) with SoftMax Pro Easy software, and the NADH/NAD + ratio was calculated by comparing the relative light units. 713579). All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. 40266 Email: drugdiscoverytech@lifetech. HERM LB 500 with NaI detector Radio HPLC Detector. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. SpectraMax iD5. Click Run. ScanLater Western Blot Detection System. A photomultiplier tube (PMT) converts a light pulse into an electrical signal of measurable magnitude. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. PerkinElmer; Revvity; Customer Transformation Hub. Equal concentrations of protein were separated in Mini-PROTEAN® TGX™ Precast 4–20% Gels (Bio-Rad, Hercules, CA, USA) and transferred to nitrocellulose. A blank sample was prepared by mixing. 4 Pages. Transfluor Technology. book Page 1 Tuesday, September 7, 2010 6:14 PM. DCF fluorescence was measured by using a microplate reader (FilterMax F5) at maximum excitation and with emission spectra of 485 nm and 535 nm, respectively. The following document is intended to demonstrate setup of this instrument. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Related Products: FilterMax F3 & F5 Multi-Mode Microplate Readers, SpectraMax i3 Multi-Mode Platform Microplate Detection System,. The results are expressed as cell viabilities (%) based on the control condition. In addition, the instrument features linear and orbital shaking, plus temperature control. Biofilm quantification and scanning electron microscopy (SEM) analysis. The Molecular Devices FilterMax F5 Multi-Mode Plate Reader was tested for compatibility with Life Technologies’ Z'-LYTE® assays using the Z'-LYTE® Tyr6 kit (PV4122) against. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. SpectraMax i3x Multi-Mode Detection Platform Biotek Synergy H1 Operator's Manual Appliskan User Manual FilterMax F3/F5 Multi- Mode Microplate Readers Spectramax® m2/m2 user guide User manual Software version 3. Important things to consider when purchasing a multimode microplate reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Read Review (1)The mixture were then centrifuged at 1000 g for 5 min. com 8. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. Accurate measurements are ensured by automatic lamp calibration prior to each reading. 25. SpectraMax Glo Steady-Luc Reporter Assay Kit. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. GPCRs. SpectraMax M-series Microplate readers. Cytokines Assay. 00 USD. In this work, we studied the activity of the antidepressant drug sertraline against Trypanosoma cruzi trypomastigotes and intracellular. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataFilterMax ™ F5 Multi-Mode Microplate Reader; SpectraMax L Microplate Luminometer; Methods. The plate was incubated for 1 hr at room temperature with shaking. Recommended Optics The lysates were incubated in caspase-3 substrate Ac-DEVD-pNA at 37 °C. 2 Temperature and cooling rate control. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. 00. ; Page 2 For research use only. Plate Formats:. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 9 mM H 2 O 2 for. 3 Pages. The amount of the colorimetric product was monitored continuously for 120 min. SP Bel-Art Petri Dish Plate Reader, Bel-Art Products, a part of SP . Read Review (1)With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Page 2 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 7. Multi-mode Microplate Readers. In addition, the instrument features linear and orbital shaking, plus temperature. , Shanghai, China) and microplate reader (FilterMax F5 Multi-Mode Microplate Reader, LLC, Molecular Devices, San Jose, CA, United States) at a wavelength of 450 nm. The drawing shows a standard microplate of 15 mm height covered with a lid. , Sunnyvale, CA, USA). The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. 2. 003 fmol 0. Supplier: Molecular Devices. 4. Gently pull the plastic tab protruding from the microplate chamber door. MetaFluor Software.